Here is a little blog, english mirror of my french version already online since 2008 and available here
I will not do a whole PCR tutorial here on this blog but only experience of what I have had as qPCR user since 1998...
And for the first post I am going to start like others can finish their own blog : with links.
Indeed, you can find enough informations about conventional PCR not to ble obliged to do it on myself.
Here are few basic links but clear to understand what is the technique and also news about the technique.
- A simple explanation : http://users.ugent.be/~avierstr/principles/pcr.html- A little movie about PCR steps : http://www.dnalc.org/ddnalc/resources/shockwave/pcranwhole.html
- Another conventional PCR movie with 3 steps : http://www.maxanim.com/genetics/PCR/PCR.htm
Next post will have links for qPCR
Waiting for the next translation time I will have to do it, here is a link very useful to have information about PCR like technical ones, HRM, molecules, statistics, supplier, cyclers and many many other informations : http://www.gene-quantification.info
Have a good amplification then.
